Light intensity effects on production and antioxidant activity of flavonoids and phenolic compounds in leaves, stems and roots of three varieties of Labisia pumila Benth
نویسندگان
چکیده
This research was performed to evaluate the effect of light intensity (310 and 630 μmol ms) on production and antioxidant activity of flavonoids and phenolic compounds from the leaves, stems and roots in three varieties of Labisia pumila Benth. The experiment was carried out base on randomized complete block design with a factorial arrangement. The results revealed that total phenolic and flavonoid content as well as antioxidant activity in all three varieties had consistently higher values when exposed to high irradiance (70% IR). The highest amount of these components was accumulated in the leaves followed by the roots and stems in all three varieties. The results showed that total flavonoid accumulation was highest in the leaves of L. pumila var pumila (2.94±0.11 mg rutin equivalent /g DW) under 630 μmol ms light intensity and total phenolics was highest in L. pumila var alata (3.92±0.06 mg Galic acid equivalent (GAE)/g DW) under the same light intensity. HPLC analyses of phenolics and flavonoids in all three varieties revealed the presence of gallic acid, caffeic acid, kaempferol, naringin and myricetin in the leaves of all three varieties of L. pumila Benth and these compounds increased under 630 μmol ms light intensity. Antioxidant activities determined by 1,1Diphenyl-2-picryl-hydrazyl (DPPH) and ferric reduction antioxidant power (FRAP) assays in all varieties. The results showed significantly (p ≤ 0.05) higher activity with increasing total phenolics and flavonoids in all plant organs under 630 μmol ms light intensity compared to 310 μmol ms. This study indicates the ability of different light intensities to enhance the secondary metabolites such as flavonoid and phenolic compounds as well as their antioxidant activities in all three varieties of L. pumila Benth.
منابع مشابه
Comparison of bioactive compounds and their antioxidant potentials of three varieties of Labisia pumila Benth. extracts obtained from different extraction methods. Ehsan Karimi1*, Hawa Z. E. Jaafar2, Pooyan MehrabanJoubani3
Extraction methods are very important processes for separation of bioactive compounds of medicinal plants before analyses. Phenolic and flavonoid compounds include a broad variety of structures and functionalities that are required for the production of foods and food additives and there are different methods for the their extraction. In this study, total phenolic and flavonoid contents of leaf...
متن کاملThe relationship of nitrogen and C/N ratio with secondary metabolites levels and antioxidant activities in three varieties of Malaysian kacip Fatimah (Labisia pumila Blume).
Kacip Fatimah (Labisia pumila Blume), one of the most famous and widely used herbs, especially in Southeast Asia, is found to have interesting bioactive compounds and displays health promoting properties. In this study, the antioxidant activities of the methanol extracts of leaves, stems and roots of three varieties of L. pumila (var. alata, pumila and lanceolata) were evaluated in an effort to...
متن کاملPhenolics and flavonoids compounds, phenylanine ammonia lyase and antioxidant activity responses to elevated CO₂ in Labisia pumila (Myrisinaceae).
A split plot 3 × 3 experiment was designed to examine the impact of three concentrations of CO₂ (400, 800 and 1,200 μmol·mol⁻¹) on the phenolic and flavonoid compound profiles, phenylalanine ammonia lyase (PAL) and antioxidant activity in three varieties of Labisia pumila Benth. (var. alata, pumila and lanceolata) after 15 weeks of exposure. HPLC analysis revealed a strong influence of increase...
متن کاملHPLC and GC-MS determination of bioactive compounds in microwave obtained extracts of three varieties of Labisia pumila Benth.
Microwave extraction of phytochemicals from medicinal plant materials has generated tremendous research interest and shown great potential. This research highlights the importance of microwave extraction in the analysis of flavonoids, isoflavonoid and phenolics and the antioxidant properties of extracts from three varieties of the Malaysian medicinal herb, Labisia pumila Benth. High and fast ex...
متن کاملThe relationship between phenolics and flavonoids production with total non structural carbohydrate and photosynthetic rate in Labisia pumila Benth. under high CO2 and nitrogen fertilization.
A factorial split plot 4 × 3 experiment was designed to examine and characterize the relationship among production of secondary metabolites (total phenolics, TP; total flavonoids, TF), carbohydrate content and photosynthesis of three varieties of the Malaysian medicinal herb Labisia pumila Benth. namely the varieties alata, pumila and lanceolata under CO(2) enrichment (1,200 µmol mol(-1)) combi...
متن کامل